An Introduction to Single Cell ICP - MS Analysis
نویسندگان
چکیده
The introduction and development of Single Particle ICP-MS (SP-ICP-MS) has opened a new area of research which allows the rapid detection and analysis of metal-based particles in a variety of matrices and applications.1-6 The key feature of SP-ICP-MS is that it allows discrete pulses of positively charged ions to be detected and measured in a time-resolved manner using microsecond (μs) data acquisition rates. This advance in data acquisition capabilities is opening up a number of new application areas. In this white paper, we will introduce the concept of Single Cell ICP-MS (SC-ICP-MS), where individual cells are rapidly analyzed for metal content. Before delving into SC-ICP-MS, let’s briefly review the theory of SP-ICP-MS.
منابع مشابه
Time-resolved ICP-MS measurement: a new method for elemental and multiparametric analysis of single cells.
Time-resolved inductively coupled plasma mass spectrometry (ICP-MS) has attracted much attention for elemental and multiparametric analysis of single cells, instead of a classical bulk analysis of large amount of cells after a dissolution. In the time-resolved measurement, cells are directly introduced into the plasma via nebulizing or micro drop dispensing, and then ion plumes corresponding to...
متن کاملQuantification and visualization of cellular uptake of TiO2 and Ag nanoparticles: comparison of different ICP-MS techniques
BACKGROUND Safety assessment of nanoparticles (NPs) requires techniques that are suitable to quantify tissue and cellular uptake of NPs. The most commonly applied techniques for this purpose are based on inductively coupled plasma mass spectrometry (ICP-MS). Here we apply and compare three different ICP-MS methods to investigate the cellular uptake of TiO2 (diameter 7 or 20 nm, respectively) an...
متن کاملBasic Performance of the Agilent 7700s ICP-MS for the Analysis of Semiconductor Samples
Agilent ICP-MS systems have become the benchmark for accurate low-level analysis of trace contaminants across a wide range of high-purity chemicals used in the semiconductor industry. As the first commercial ICP-MS to offer reliable and routine cool plasma operation, the Agilent 4500 Series ICP-MS set the standard for low level analysis of the previously problematic elements Na, K, Ca and Fe. B...
متن کاملDevelopments in ICP-MS: electrochemically modulated liquid chromatography for the clean-up of ICP-MS blanks and reduction of matrix effects by flow injection ICP-MS
v Chapter 1. General Introduction 1 Historical Perspectives 1 Fundamental Aspects of ICP-MS 2 ICP-MS Instrumentation 3 Blank Purity in ICP-MS 4 Previous EMLC Experiments 6 Matrix Effects in ICP-MS 7 Flow Injection in ICP-MS 9 Shielded Torch in ICP-MS 10 Dissertation Objectives and Organization 11 Figures 12 References 27 Chapter 2. Electrochemically-Modulated Liquid Chromatography for Cleaning ...
متن کاملDirect Measurement of Metallic Impurities in 20% Ammonium Hydroxide by 7700s/7900 ICP-MS
Ammonium hydroxide (NH4OH) is a chemical used in the manufacture of semiconductor devices, and must therefore be analyzed for trace metal impurities. The direct analysis of undiluted (20%) NH4OH using ICP-MS is challenging because its high vapor pressure causes plasma instability. However, dilution of the samples would degrade detection limits, so the ability to directly analyze 20% NH4OH is im...
متن کامل